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KMID : 0380019940090020230
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Korean Journal of Biotechnology and Bioengineering
1994 Volume.9 No. 2 p.230 ~ p.236
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Mathematical Analysis of a High Density Animal Cell Culture with a Spin-Filter
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Abstract
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Spin-filters are used as cell separation devices for achieving high cell density and high productivity in animal cell culture. We have proposed a model for the cell growth in a spin-filter perfusion culture and examined the effects on cell growth by several parameters including ammonia inhibition, specific growth rate, specific feeding rate, and cell retention. Results from computer simulation and sensitivity analysis indicate that the cell retention affects the cell growth mostly while there is a significant inhibition on cell growth by the ammonia accumulated during the culture. ´e specific feeding rate has minimal effects on cell growth, which is consistant with the fact that the cell growth with a step feeding is quite similar to that with a continuous feeding.
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